MCQs Test Identification of Bacteria

MCQs Test on Identification of Bacteria

Test

 

1. This stain is mainly used for negative staining.
   1.   Nigrosin
   2.   Crystal violet
   3.   Methylene Blue
   4.   Methyl Blue
2. Which of the following is a Differential staining technique
   1.   Simple Staining.
   2.   Capsule Staining
   3.   Acid Fast Staining
   4.   Spore STaining
3. Gram negative bacteria are generally classified and identified by…
   
   1.   Urease Test
   2.   Catalase Test
   3.   Oxidase Test
   4.   IMViC Test
4. Which of the following organism is Acid Fast,
   1.   Mycobacterium leprae
   2.   E. Coli
   3.   P. aeruginosa
   4.   S. typhi
5. What is the correct sequence of staining reagents in Gram-Staining?
   1.   Crystal violet, ,safranin, iodine solution, alcohol
   2.   Crystal violet, iodine solution, alcohol, safranin
   3.   Crystal violet, safranin, alcohol, iodine solution
   4.   Iodine solution, crystal violet, alcohol, safranin
6. Which bacteria appears purple/violet colour after Gram staining?
   1.   Gram-negative
   2.   Gram-positive
   3.   Both Gram-positive and Gram-negative
   4.   None
7. Which of the following are true for Gram-negative bacteria?
   1.   a) upon alcohol treatment, the permeability of the cell wall increases
   2.   crystal violet-iodine (CV-I) complex is extracted
   3.   pore size decreases and the CV-I complex cannot be extracted
   4.   alcohol treatment increases the permeability of the cell wall and the CV-I complex can be extracted
8. Gram-positive bacteria are usually more susceptible to which of the following antibiotics.
   
   1.   streptomycin
   2.   tetracycline
   3.   penicillin
   4.   Kanamycin
9. In Gram-staining, iodine is used as a ______________
   1.   fixative
   2.   mordant
   3.   solubilizer
   4.   stain
10. Which of the following is the example of Gram-negative bacteria?
    1.   Lactobacillus
    2.   Eschericia coli
    3.   Staphylococcus aureus
    4.   Bacillus subtilis
11. Gram staining was developed by
    1.   Christian Gram
    2.   Charlie Gram
    3.   Louis Pasteur
    4.   Antony Leeuwenhoek
12. Gram staining is an example of,
    1.   Simple Staining.
    2.   Differential Staining.
    3.   Special Staining
    4.   Acid Fast Staining
13. The primary stain used in Gram staining is
    1.   Safranin
    2.   Crystal Violet.
    3.   Malachite green.
    4.   Nigrosin
14. The counterstain used in Gram staining is,
    1.   Safranin
    2.   Crystal Violet.
    3.   Malachite green
    4.   Nigrosin
15. The primary stain used in Acid Fast staining is,
    1.   Carbol fuschin.
    2.   Crystal Violet.
    3.   Malachite green.
    4.   Nigrosin
16. The counterstain used in Acid Fast staining is,
    
    1.   Safranin
    2.   Crystal Violet
    3.   Malachite green
    4.   Nigrosin
17. The counterstain used in Acid Fast staining is,
    
    1.   Safranin
    2.   Crystal Violet
    3.   Methylene blue
    4.   Nigrosin
18. Which of the following statements is incorrect for gram -ve bacteria
    1.   cell wall has a thin peptidoglycan layer
    2.   lipopolysaccharide are present in large quantities.
    3.   Lipid content of the cell wall is lesser.
    4.   All of the above.
19. In direct staining,
    1.   Organism is stained and the background remains transparent.
    2.   Background is stained and the organism remains transparent.
20. In negative staining,
    1.   Organism is stained and the background remains transparent.
    2.   Background is stained and the organism remains transparent.